The first session of reunions is scheduled for 3 p.m., followed by a dinner hosted by the North Korean side.
On their second day, they are scheduled to meet again in the morning and have lunch together in their rooms, the first time for separated families to have such a private meeting since the reunion event started.
The South Korean participants are mostly in their 70s and 80s, with a 101-year-old man the oldest.
The Seoul government has also dispatched around 30 medical staff to the venue to brace for any emergency cases among the participants.
From Friday to Sunday, 83 North Koreans will also be reunited with the families they have found alive in the South at the resort.
The family reunion is the result of an agreement the leaders of South and North Korea reached in April to address humanitarian issues arising from nearly seven decades of division caused by the Korean War, Yonhap reported.
The two Koreas have held 20 rounds of face-to-face family reunions since the first-ever inter-Korean summit in 2000.
